PEMISAHAN MINYAK ATSIRI RIMPANG JAHE (Zingiber officinale Rosc) SECARA KROMATOGRAFI LAPIS TIPIS DAN AKTIVITASNYA TERHADAP JAMUR Malassezia furfur IN VITRO

Abstract

Background : Nowadays people tend to use natural ingredients as a form of alternative medication, one of them Zingiber officinale, a kind of Zingiberaceae, believed as a medication for many diseases. As reported from the previous research, essential oil of Zingiber officinale had an antifungal effect on Klebsiella pneumonia and it’s extract affected on Candida albicans. Objective : The purpose of this study was to know chromatogram profile and antifungal activity of Zingiber officinale for Mallassezia furfur in vitro. Method : This study was experimental study using the “post test only control group design”. Sample was essential oil of Zingiber officinale isolated by thin layer chromatograpy and Malassezia furfur with standard Mc Farland 0,5. The Silica Gel GF 254 as stationer phase and Chloroform benzene as mobile phase with 1:1 equation and UV light 254 nm as a spot clearing was used and to check antifungal activity, it was divided into 5 groups and a control groups. The test groups mixed with the different dose of Zingiber officinale from 6,25 % v/v until 100 % v/v. And the control group was positive control. Incubation was done for 2 days at 37o temperatures. Evaluation was carried on by seeing fungus’s growth in medium’s surface. Result : The results of analysis using thin layer chromatography was found 4 spot. Antifungal activity determine after Kruskal-Wallis Test was done, the result was significant (p<0,05) and followed by Mann-Whitney Test, significant all of the doses. Conclusions : Zingiber officinale has four compoundsn that probably belonged to terpen’s group. Antifungal Zingiber officinale againts Malassezia furfur efectiv from concentration 6,25 % v/v. Keywords : Essential oil, Zingiber officinale Rosc, Malassezia furfur