The Effects of Sponge Haliclona sp and Gorgonian Isis hippuris Combination Extract on Histopathological Feature of Swiss Mice Liver

Abstract

Backgrounds: Sponge Haliclona sp and Gorgonian Isis hippuris are marine organisms whose extracts are potential for anticancer. Nevertheless, there is only little information on both extracts toxicities which signifies subchronic toxicity study in this research. Histopathological feature of liver was chosen as an outcome concerning its role in body protection against toxicants. The purpose of this study was to investigate the effects of both extract’s combination in graded doses on histopathological feature of Swiss mice liver after 3 months treatment. Methods: This experimental study utilized post test only control group design. Fifty Swiss mice (male and female) were divided into 5 groups, treatment groups (T1, T2 and T3) were receiving 500 mg of food pellets that contained 0,15 mg; 1,5 mg; and 15 mg combination extract respectively. And as the control groups, C0 received food pellets contained merely ethanol while C negative received no treatment. Liver histopathology were observed and scored by modification of Knodell scoring system after 3 months treatment. Kruskal Wallis test was applied for all data. Results: There were insignificant differences between each group with p value 0,914. Conclusion: Sponge Haliclona sp and Gorgonian Isis hippuris combination extract caused no note-worthy liver damage after 3 months treatment in mice. Key words: Sponge Haliclona sp, Gorgonian Isis hippuris, liver histopathological feature, toxicity, anticancer, subchronic