PERBANDINGAN EFEK ANTIBAKTERI JUS NANAS (Ananas comosus L.merr) PADA BERBAGAI KONSENTRASI TERHADAP Streptococcus mutans

Abstract

Background: A high prevalence of dental caries is a problem of health in Indonesia for along time, and Streptococcus mutans is considered as one of the etiological factor. The complexity of pineapple’s contents which has an ability to decrese plaque forming, is supposed to be able to lower the prevalence of dental caries. Objective: The purpose of this study was to measure the antibacterial effect of pinepple juice on S. mutans in vitro. Metode: This study was an experimental study using post test only control group design. The method used for antibacterial effect test was dilution methode. They were devided into four test groups and three control groups. The test groups were treated with differrent concentration of pineapple juice of 100%, 50%, 25%, and 12,5%. The control groups acted as sample control, negative control, positive control. Minimum Inhibitory Concentrations (MIC) was determined by visual clearity of pineapple juice on Brain Heart Infusion Broth (BHIB), whereas Minimum Bactericidal Concentrations (MBC) was determined by finding of S. mutans colonies in Blood Agar (BA). Statistic analysis was done by Kruskal-Wallis Test and followed by Mann-Whitney Test. Result: Pineapple juice could inhibited S. mutans at 25%, as well as eliminated them at 100% of concentrations. Conclusion: Pineapple juice has bacteriostatic and bactericidal effects on S. mutans. MIC for S. mutans was 25% and MBC was 100%. Keywords: Pineapple juice (Ananas comosus L.merr), Streptococcus mutans, Antibacterial effect, Minimum inhibitory Concentrations (MIC), Minimum Bactericidal Concentrations (MBC).