Efek Paparan Arus Listrik terhadap Jumlah Titik Hiperkontraksi Otot Gastrocnemius dan Kadar Kreatin Kinase Serum Tikus Wistar

Abstract

ABSTRAK Latar belakang : Tujuan penelitian ini adalah untuk membuktikan efek paparan arus listrik terhadap jumlah titik hiperkontraksi serabut otot gastrocnemius dan kadar kreatin kinase serum. Metode : 54 ekor tikus wistar diambil dengan simple random sampling. Sampel dibagi menjadi 9 kelompok, yaitu: kelompok 1-4 terpapar arus listrik secara langsung,yaitu berturut-turut 1-30 mA, 31-60 mA, 61-90 mA, 91-120 mA. Kelompok 5-8 terpapar arus listrik melalui medium air, yaitu berturut-turut 1-30 mA, 31-60 mA, 61-90 mA, 91-120 mA. Kelompok 9 tidak terpapar arus listrik. Setelah adaptasi selama 7 hari, dilakukan paparan arus listrik kemudian dilakukan pemeriksaan kadar kreatin kinase serum dan pemeriksaan jumlah titik hiperkontraksi serabut otot gastrocnemius. Analisis data dilakukan dengan Uji Spearman Hasil : Terdapat korelasi positif antara besar paparan arus listrik secara langsung dan melalui medium air dengan kadar kreatin kinase serum tikus wistar (rho = 0,632; p = 0,000, dan rho = 0,701; p = 0,000). Terdapat korelasi positif antara besar paparan arus listrik secara langsung dan melalui medium air dengan jumlah titik hiperkontraksi serabut otot gastrocnemius ( rho = 0,959; p = 0,000, dan rho = 0,910; p = 0,000) Simpulan : Semakin besar paparan arus listrik, semakin besar jumlah hiperkontraksi serabut otot gastrocnemius dan kadar kreatin kinase serum. Kata kunci : arus listrik, kontak langsung, medium air, otot gastrocnemius, hiperkontraksi, kreatin kinase ABSTRACT Background: This study aim to prove the effect of electric current in graded doses towards hypercontraction on gastrocnemius level and creatine kinase serum level of wistar rats. Method: 54 wistar rats were divided into 9 groups through simple random sampling. Group 1-4 received electric current through direct contact, while group 5-8 received it through water conduction. Group 1 and 5, 2 and 6, 3 and 7, 4 and 8 received 1-30 mA, 31-60 mA, 61-90 mA, and 91-120 mA of electric current respectively. Group 9 was received no treatment. After adaption period for 7 days, electrocution was performed, and then creatine kinase serum level was measured and assessed the amount of hypercontraction on gastrocnemius muscle. Statistical analysis was conducted by using Spearman test Result: There was significant positive correlation between electric current in graded doses and creatine kinase serum level both through direct contact and water conduction (rho = 0.632, p = 0.000; and rho = 0.701, p = 0.000 respectively). There was significant positive correlation between electric current in graded doses and hypercontraction on gastrocnemius muscle both through direct contact and water conduction (rho = 0.959, p = 0.000; and rho = 0.910, p = 0.000 respectively). Conclusion: Higher doses of electric current correlated more numbers of hypercontraction on gastrocnemius muscle and higher level of creatine kinase serum. Keywords: electric current, direct contact, water conduction, gastrocnemius muscle, hypercontraction, kreatin kinase .