Efek Paparan Arus Listrik terhadap Jumlah Titik Hiperkontraksi Otot Gastrocnemius dan Kadar Kreatin Kinase Serum Tikus Wistar The Effect of Electric Current towards Hypercontraction on Gastrocnemius muscles and Creatine Kinase Serum Level of Wistar Rats

Abstract

ABSTRAK Latar belakang : efek paparan arus listrik diperkirakan melalui hiperkontraksi serabut otot gastrocnemius dan kadar kreatin kinase serum. Tujuan penelitian ini adalah untuk membuktikan efek paparan arus listrik terhadap jumlah titik hiperkontraksi serabut otot gastrocnemius dan kadar kreatin kinase serum. Metode : 54 ekor tikus wistar diambil dengan simple random sampling. Sampel dibagi menjadi 9 kelompok, yaitu: kelompok 1-4 terpapar arus listrik secara langsung,yaitu berturut-turut 1-30 mA, 31-60 mA, 61-90 mA, 91-120 mA. Kelompok 5-8 terpapar arus listrik melalui medium air, yaitu berturut-turut 1-30 mA, 31-60 mA, 61-90 mA, 91-120 mA. Kelompok 9 tidak terpapar arus listrik. Setelah adaptasi selama 7 hari, dilakukan paparan arus listrik kemudian dilakukan pemeriksaan kadar kreatin kinase serum dari plexus retro orbitalis dengan COBAS Integra 400 Plus-Roche. Setelah dekapitasi dilanjutkan dengan pemeriksaan jumlah titik hiperkontraksi serabut otot gastrocnemius. Analisis data dilakukan dengan Uji Spearman Hasil : Terdapat korelasi positif antara besar paparan arus listrik secara langsung dan melalui medium air dengan kadar kreatin kinase serum tikus wistar (rho = 0,632; p = 0,000, dan rho = 0,701; p = 0,000). Terdapat korelasi positif antara besar paparan arus listrik secara langsung dan melalui medium air dengan jumlah titik hiperkontraksi serabut otot gastrocnemius ( rho = 0,959; p = 0,000, dan rho = 0,910; p = 0,000) Simpulan : Semakin besar paparan arus listrik, semakin besar jumlah hiperkontraksi serabut otot gastrocnemius dan kadar kreatin kinase serum. Kata kunci : arus listrik, kontak langsung, medium air, otot gastrocnemius, hiperkontraksi, kreatin kinase ABSTRACT Background: Electric current is assumed to cause hypercontraction on gastrocnemius muscles and disturbance of creatine kinase serum level of Wistar rats. This study aim to prove the effect of electric current in graded doses towards hypercontraction on gastrocnemius level and creatine kinase serum level of wistar rats. Method: 54 wistar rats were divided into 9 groups through simple random sampling. Group 1-4 received electric current through direct contact, while group 5-8 received it through water conduction. Group 1 and 5, 2 and 6, 3 and 7, 4 and 8 received 1-30 mA, 31-60 mA, 61-90 mA, and 91-120 mA of electric current respectively. Group 9 was received no treatment. After adaption period for 7 days, electrocution was performed, and then creatine kinase serum level was measured from plexus retro orbitalis by using COBAS Integra 400 Plus-Roche. Rats were then decapitated in order to assess the amount of hypercontraction on gastrocnemius muscle. Statistical analysis was conducted by using Spearman test Result: There was significant positive correlation between electric current in graded doses and creatine kinase serum level both through direct contact and water conduction (rho = 0.632, p = 0.000; and rho = 0.701, p = 0.000 respectively). There was significant positive correlation between electric current in graded doses and hypercontraction on gastrocnemius muscle both through direct contact and water conduction (rho = 0.959, p = 0.000; and rho = 0.910, p = 0.000 respectively). Conclusion: Higher doses of electric current correlated more numbers of hypercontraction on gastrocnemius muscle and higher level of creatine kinase serum. Keywords: electric current, direct contact, water conduction, gastrocnemius muscle, hypercontraction, kreatin kinase .