PENENTUAN LD50 EKSTRAK ETANOL KULIT AKAR SENGGUGU (Clerodendron serratum Spreng)TERHADAP MENCIT BALB/C

Abstract

Background : Senggugu (Clerodendron serratum Spreng.) is a plant that is use to stimulate the secretion of mucous (“gurah”). From previous study it is shown that Senggugu root bark has mucolitic effect on mucous gland by decreasing mucous viscosity. Phenolglycoside, mannitol and sitosterol are the substances that is verified to be found in Senggugu root bark. Senggugu is widely applied among Indonesian community for ages but the toxicity has not yet been determined. Therefore it is important to measure the LD-50 value to decide toxicity potential of Senggugu root bark ethanolic extract. Objective : The aim of this research was to assess the potency of acute toxicity, evaluate clinical symptoms and spectrum of toxic effect of experimental animals after administration of Senggugu (Clerodendron serratum Spreng.) root bark ethanolic extract to Balb/C male mice. Method : This research was an experimental study used post test only control group design. The experimental objects were Balb/C male mice, healthy, age between 2-3 months old with identical body weight (25-30 g). Twenty-four Balb/C mice samples were divided into four groups, that was six mice for each group, including one control group and three experimental groups. The Senggugu root bark ethanolic extract dosage that was given for each group were 1 g of body weight; 1,5 g of body weight; and 2 g of body weight. The symptoms of toxicity and death of experimental subject was evaluated for seven days in a row. Result : This research obtained no dead mice found between the observation period. The extract administration caused lacrimation, besides alteration of animal behaviours including passiveness. Conclusion : LD50 value of ethanolic extract from Senggugu root bark was cannot be determined on Balb/C male mice. Key words : Clerodendron serratum Spreng., LD50, Balb/C male mice