UJI AKTIVITAS ANTIBAKTERI EKSTRAK BAWANG PUTIH ( Allium sativum Linn ) TERHADAP BAKTERI Escherichia coli IN VITRO

Abstract

Background: Allium sativum Linn is one medicine plants known by people for a long time. It has been known for its antibacterial effect. Objective: The purpose of this study was to know antibacterial activity of Allium sativum Linn extract against Escherichia coli. Method: This research was an experimental study with post test control group only design. The sample of this experimental study was extract of Allium sativum Linn used maceration method. The antibacterial activity test uses dilution method, these were divided into 10 test group with concentration 100% v/v, 50% v/v, 25% v/v, 12,5% v/v, 6,25% v/v, 3,125% v/v, 1,56% v/v, 0,78% v/v, 0,39% v/v, 0,19% v/v, and 3 control groups were positive control, negative control, and sample control. Statistic analysis was done by using Kruskal-Wallis Test and Mann-Whitney Test. Result: The result of antibacterial activity, Minimum Inhibitory Concentration (MIC) significant from the concentration 50% v/v (p=0,008). Minimum Bactericidal Concentration (MBC) significant from the concentration 50% v/v (p=0,008). Conclusion: The result of antibacterial activity test were bactericidal for Escherichia coli. MIC and MBC for Escherichia coli was 50% v/v. Keyword: Allium sativum Linn, Escherichia coli, antibacterial effect, MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration).