UJI AKTIVITAS ANTIBAKTERI EKSTRAK BAWANG PUTIH ( Allium sativum Linn ) TERHADAP BAKTERI Staphylococcus aureus IN VITRO

Abstract

Backround : Allium sativum is a well know advantageous herbal. Previous studies show that A. sativum had antibacterial effect to Staphylococcus aureus Objective : The purpose of this research was to measure the antibacterial effect of A. sativum extract againt Staphylococcus aureus. Method : This research was an experimental study with post test control group only design. The sample of this experimental study was extract of Allium sativum Linn used maceration method. The antibacterial activity test uses dilution method, these were divided into 10 test group with concentration 100% v/v, 50% v/v, 25% v/v, 12,5% v/v, 6,25% v/v, 3,125% v/v, 1,56% v/v, 0,78% v/v, 0,39% v/v, 0,19% v/v, and 3 control groups were positive control, negative control, and sample control. MIC was determined by visual clarity to prevent Mueller Hinton. MBC was determined by ability to prevent Staphylococcus aureus from growing on Mueller Hinton Agar. Statistic analysis was done by using Kruskal-Wallis Test and Mann-Whitney test Result : Consentration of 12,5% significantly inhibited bacterial growth compared to positive control. All samples did not show different ability in bactericidal activity Conclusion : MIC of A. sativum to Staphylococcus aureus is 12,5%. MBC of Allium sativum to Staphylococcus aureus could not be determined by this study Suggestion : Another study with higher concentration is needed to determine MBC of A. sativum to Staphylococcus aureus Keyword : Allium sativum, Staphylococcus aureus, antibacterial effect, MIC (Minimum Inhibitory Concentration, MBC (Minimum Bactericidal Concentration)