PENGARUH PEMBERIAN TEH HIJAU TERHADAP KADAR ENZIM GAMMA GLUTAMYL TRANSFERASE SERUM TIKUS WISTAR YANG DIBERI KLORAMFENIKOL

Abstract

Background : Gamma Glutamyl Transferase enzyme is a specific excretoric enzyme which indicates liver damage. Chloramphenicol is a poten antibiotic for typhoid fever that has effect on the liver. Green tea is one of the popular beverage in community. Its active component, polifenol, to protectst human body cells from free radical effects, including liver damage. Objective : To know the effect of green tea administration on the decrease of serum gamma glutamyl transferase level Wistar rats given with chloramphenicol. Methods : This study was an experimental study by using Post Test Only Control Group Design. Samples were female Wistar rats, healthy, 6 weeks old and weighted ± 125 mg. Samples were divided randomly into three groups. The control group, each rat was given water from day 6 to 22. In the first treatment group, each rat was given chloramphenicol 25 mg/kgBB from day 6 to 22. In the second treatment group, each rat was given boiled green tea 330 mg per day with chloramphenicol 25 mg/kg weight from day 6 to 22. On day 23, blood from abdominal vein was taken to measure the Gamma GT serum. The data were processed by SPSS 15.00 for Windows, using Saphiro-Wilk test and One Way Anova test. Results : Saphiro-Wilk test showed Gamma GT value serum showed a normal distribution in the serum Gamma GT level (p<0.05). And One Way Anova test showed that there was no decrease of serum Gamma GT serum value significantly, p=0.297 (p>0.05). Conclusions : Then result indicated that green tea administration could not decrease the Gamma Glutamyl Transferase serum level in wistar rats given with chloramphenicol. Keyword : Green tea, Gamma GT, Chloramphenicol.