EFEK MINYAK ATSIRI DARI Allium sativum DAN Piper retrofractum Vahl. TERHADAP HDL : (Studi Eksperimental pada Serum Tikus Wistar yang Diberi Diet Kuning Telur Intermiten)

Abstract

Background : Garlic’s (Allium sativum) essential oils contains diallyl disulfide (DADS). Long pepper’s (Piper retrofractum Vahl.) essential oils contains terpenoid. Many literature said that both compound could reduce cholesterol by inhibiting HMG-CoA reductase enzyme. The aim of this research is to find out the effect of combination garlic’s essential oils and long pepper’s essential oil to cholesterol HDL level than the single use of them. Methods : The design of this experimental study was randomized post-test only control group design. The samples were twenty eight male Wistar, 8 weeks which got egg yolk diets intermittenttly. The samples were divided into 4 groups, one group was control group (K) and the other was treatment group (P1, P2, P3). All groups were given 1,5 gram egg yolk diets intermittently for 2 weeks. After that the control group (K) was given standard diets, the second group (P1) was given garlic’s essential oils, the third group (P2) was given long pepper’s essential oils, and the last group (P3) was given combination of garlic’s essential oils and long pepper’s essential oils for 3 weeks. The dose of essential oils was 0,05 ml. The data were tested with One Way Anova. Result : Cholesterol HDL level of treatment group P1 (42,41±10,25); P2 (43,59±10,43); and P3 (36,40±6,86) are lower than control group (45,47±9,69). One Way Anova test between control group and treatment group was not significantly different (p=0,326, p>0,05). Conclusion: Cholesterol HDL serum level were not rise in the giving of essential oil from Allium sativum and Piper retrofractum Vahl. at 0,05 ml dosage for 3 weeks to eight weeks male wistar rats. Keywords: Essential oil, Allium sativum, Piper retrofractum Vahl., cholesterol HDL serum