Pengaruh Pemberian Teh Hijau terhadap Fungsi Hati Tikus Wistar yang Diberi Kloramfenikol

Abstract

Background : Catechin, which is an essential component in green tea, can stimulate the activity of P450 cytochrome enzyme, therefore it prevents the accumulation of reactive metabolites that can damage the hepatocytes. Administration of Chloramphenicol can cause liver damage by suppressing the activity of that enzyme. The liver damage causes release of intra hepatocyte enzymes and other indicators to the blood circulation. Objectives : The objective of this experiment is to find the effect of administration of green tea to liver function of Wistar Rat that was given Chloramphenicol by measuring the serum number of Aspartate Amino Transferase, Alanin Amino Transferase, Gamma-GT, Alkaline Phosphatase, direct Bilirubin, indirect Bilirubin, and total Bilirubin. Method : This research was an experimental research which used The Post Test Only Control Group Design. The sample population of this experiment were obtained from healthy female Wistar rats, about 6 weeks old, and + 125 mg in weight. The samples were divided randomly into three groups : control group (K) where each rat was given physiologic NaCl; treatment group 1 (P1) where each rat was given physiologic NaCl and added with Chloramphenicol 25 mg / kg weight; and treatment group 2 (P2) was given boiled water of green tea twice per day 165 mg per administration and also given Chloramphenicol 25 mg / kg weight. On the 23rd day, we take the rat’s blood from the Abdominal Vein to measure the serum level of liver damage indicators in laboratory. Result : The result of this experiment shows that the serum level of SGOT decreased significantly, the serum levels of total Bilirubin, SGPT, Alkaline Phosphatase, and direct Bilirubin was not influenced. The administration of Chloramphenicol decreased the serum level of direct Bilirubin, indirect Bilirubin, and Gamma-GT. Conclusion : The administration of 165 mg green tea per day to Wistar female rat for 21 days can not improve the liver function of Wistar rat that was given Chloramphenicol, because it was only found that there is decrease of the serum level of SGOT significantly. Keywords : green tea, Catechin, SGOT, SGPT, Gamma-GT, Alkali Phosphatase, direct Bilirubin, indirect Bilirubin, total Bilirubin, Chloramphenicol.