UJI TOKSISITAS AKUT MONOCROTOPHOS DOSIS BERTINGKAT PER ORAL DILIHAT DARI GAMBARAN HISTOPATOLOGIS OTAK BESAR MENCIT BALB/C

Abstract

Background: Monocrotophos is one kind of pesticide in organophosphate group. It is very dangerous and can be found easily in Indonesia. Its effect is inhibition of cholinesterase enzyme that causes the amount of asetylcholine increase. Poisoning occure in all exposure routes, no exception in cerebrum. This study is aimed to proved the effects of acute toxicity of monocrotophos on histopathologic appearance of Balb/c mice cerebrum. Method: Experimental study with post test only control group design. The samples were 20 Balb/c mice, divided randomly into 4 groups and fulfilled all criterias. K was control group and was not given monocrotophos, P1 was given monocrotophos 1/10 x 15 mg/kgBB, P2 was given monocrotophos 15 mg/kgBB, and P3 was given monocrotophos 10 x 15 mg/kgBB. After 7th days all cerebrum sample were taken out to identify changes in histopathologic. Data was described in table and picture, statistical analysis used SPSS for Windows 18.0. Result: The highest mean of total cerebrum cells damage was in P3 group. The ANOVA test showed significant difference (0,000). The Post Hoc test showed significant difference in K-P2 (p=0,000), K-P3 (p=0,00,), P1-P2 (p=0,000), P1-P3 (p=0,000), P2-P3 (p=0,000). Conclusion: The administered of graded monocrotophos caused change on histopathologic appearance of Balb/c mice cerebrum. The histopathological change in cerebrum was shown as brain cells necrosis. Keywords: Monocrotophos, cerebrum