UJI TOKSISITAS AKUT MONOCROTOPHOS DOSIS BERTINGKAT PER ORAL DILIHAT DARI GAMBARAN HISTOPATOLOGIS OTOT JANTUNG MENCIT BALB/C

Abstract

Background: Monocrotophos is one type of organophosphate pesticides. If monocrotophos used inappropriately, it may be dangerous and cause death from respiratory failure due, This study aims to prove the relationship between graded monocrotophos with histopatologic appearence of mice heart muscle. Methods: This study design was post test only control group design. Using 4 groups with 5 sample of mice balb/c each groups which fullfilled all criterias in the laboratory of Biochemistry Faculty of Medicine Diponegoro University. K was a control group without given monocrotophos, P1 was given monocrotophos 1/10 x 15 mg/kgBB, P2 was given monocrotophos 15 mg/kgBB, and P3 was given monocrotophos 10 x 15 mg/kgBB. After the seventh day all samples heart organ were taken for later heart organ histopathologic examination. The data decribed in the form of table and picture. Shapiro wilk normality test was given, if data distribution were normal, used diffentrial test with anova followed by using statistic test with Independent T-test, if data distribution were not normal, used differential test Kruskal Wallis, followed by using statistic test with Mann Whitney using SPSS 18.0 for Windows. Results: Shapiro wilk normality test with p=0.000. Differential test Kruskal Wallis with p=0.000. Non parametrik test Mann Whitney with p=0.000 for K with P1, K with P2, K with P3, P1 with P2, P1 with P3, and P2 with P3. Logistic regression analyzed p value <0:05 (significant) for all variables. Conclusion: The administered of graded monocrotophos affect for histopatological change of mice heart muscle damage. Histopathologic changes are directly proportional to the dose of monocrotophos. Keywords: monocrotophos, histopatological appearence mice heart muscle,