PENGARUH PEMBERIAN EKSTRAK DAUN CYCLEA BARBATA L. MIERS TERHADAP MOTILITAS SPERMATOZOA MENCIT BALB/C JANTAN YANG DIPAPAR ASAP ROKOK

Abstract

Background: Cyclea barbata L. Miers is proven to contain phenol having function of as antioxidant. Antioxidant has the function of reducing the effectof free radicals. Cigarette can be one of free radicals that can reduce the amount of sperm. This research has the objective of proving the influence of the application of antioxidant contained in Cyclea barbata L. Miers on the amount of spermatozoa of the male Balb/c mice exposed to cigarette smoke. Method: The design of this research is the post test only control group design, using the Balb/c strain mice as samples. Thirty-five mice fulfilled the inclusive and exclusive criteria, devided into five treatment groups; two control groups, which are negative control (no treatment) and positive control (cigarette smoke applied), and three treatment groups applied with cigarette smoke plus stepped dosages of Cyclea barbata L. Miers, which are 3,75g/l, 7,5g/l, and 15g/l. Examination of the amount of sperm in every sample is conducted using the counting chamber. The data are desribed in from of tables. One Way Anova, and Post Hoct Test, which is LSD, are conducted using SPSS 13 for windows. Result: The Average amount of spermatozoa are: Control group (-)=13,6; Control group (+)=5,2; Treatment 1 group=8,1; Treatment 2 group=8,6; and Treatment 3 group=6,4. The One Way Anova Test, p=0,0, meaning that data there are significant differences in all groups. From the LSD test, there are significant differences between C(-) and all groups and C(+) and T1 and T2. Conclusion: The application of Cyclea barbata L. Miers leaf extract is proven to influence the amount of sperm due to the exposition to cigarette smoke. From the research results, it can be seen that the 7,5g/l dosage has the better effect compared to the 3,75g/l and 15g/l. Keywords: cigarette smoke, Cyclea barbata L. Miers, amount of spermatozoa