PENGARUH LAMA PEMBERIAN METANOL 50 % PER ORAL TERHADAP TINGKAT KERUSAKAN SEL HEPAR PADA TIKUS WISTAR

Abstract

Background: Methanol is very poisonous chemical substance and often malused for make oplosan. Some peoples show duration of death difference after consum in same doses. Many cases is reported, but study about methanol toxicity hasn’t done yet in Indonesia. Liver is main place for methanol metabolism, thus the chance of this organ getting damage is very high. This study is aimed to know the effect duration of giving methanol 50% per oral to the degradation level of liver cell in Wistar rats. Method: This experimental study used post test only controlled group design, with 20 Wistar male rats, randomly divided into 4 groups. K was control group which was only given aquadest. P1, P2, and P3 were treatment groups which were given methanol 50% 6 ml/kgBW during 5, 10, and 15 days. Methanol was orally given with sonde on the first day. At 5th,10th, and 15th day, the rats were terminated in accordance with treatment groups, and the liver was observed histopathologically. Result: The highest degradation level score of liver cell was in P3 group, which had mean value 3,02. The score evaluated parenchymatous degeneration, hydropic degeneration, and necrosis. The ANOVA test showed significant difference (p=0,000). Continued with Post Hoc test that showed significant difference in K-P1 (0,019), K-P2 (p=0,000), K-P3 (p=0,000), P1-P2 (p=0,000), P1-P3 (p=0,000), and P2-P3 (p=0,011). Conclusion: Giving methanol 50% orally to Wistar rats during 5, 10, and 15 days cause damage to liver cell as parenchymatous degeneration, hydropic degeneration, and necrosis. Keywords: methanol, liver cell degradation