PENGARUH PEMBERIAN EKSTRAK DAUN CYCLEA BARBATA L. MIERS TERHADAP MOTILITAS SPERMATOZOA MENCIT BALB/C JANTAN YANG DIPAPAR ASAP ROKOK

Abstract

Background: Cigarette smoke is a free radical that can disturb human spermatozoa such as the abnormality of membrane and sperm motility. Fenol substance in Cyclea barbata L. Miers have function as antioxidant which can stop free radical chain in lipid peroxidase. This research was directed to prove the effect of extract Cyclea barbata L. Miers to the sperm motility mice Balb/c with cigarette smoke. Methodhs: This experimental research used the post test only control group design. The sample were 35 Balb/c mice which devided into five groups. Control negative goup was only given meal standard. Control positive exposed by cigarette smoke only. Group P1 exposed by cigarette smoke and extract Cyclea barbata L. Miers 3,75 g/l/day. Group P2 exposed by cigarette smoke and extract Cyclea barbata L. Miers 7,5 g/l/day. Group P3 exposed by cigarette smoke and extract Cyclea barbata L. Miers 15 g/l/day. This experimental lasted for 21 days. On the 22th all mice were terminated and the sperm motility was examined. Result: Mean of spem motility: group K(-)=74,94; group K(+)=10,64; Group P1=45,80; Group P2=53,40; Group P3=39,54. Kruskal Wallis test shown significant difference spem motility in all group (p<0,05). Man whitney test also shown significant difference between K(-) with K(+), K(+) with P1 and K(+) with P2. Conclusion: Extract Cyclea barbata L. Miers giving effect to the sperm motility Balb/c mice with cigarette smoke significant. Dose 7,5 g/l/day give the best effect in repair and increasing sperm motility. Keywords: cigarette smoke, sperm motility, Cyclea barbata L. Miers