PENGARUH PEMBERIAN DEKSAMETASON DOSIS BERTINGKAT PER ORAL 30 HARI TERHADAP KERUSAKAN TUBULUS GINJAL TIKUS WISTAR

Abstract

Background: Dexamethasone is a synthetic corticosteroid most powerful one often used in treatment. However, as is well known the use of dexamethasone can lead to increased osmolality permeability of brush border membrane in kidney and body fluid retention leading to renal tract obstruction. This study aims to determine the effect of giving rise per oral dose of dexamethasone 30 days of renal histological features of Wistar rats and to know the minimum dose that can cause kidney damage. Methods: This was an experimental research study with the approach of The Post Test Only Control Group Design. The sample of 20 male Wistar rats were divided into four groups randomly,Tthe control group who were given only food and beverage standards, group P1 given dexamethasone orally with sonde 0.018 mg / day, a given group P2 0.731 mg / day, and group P3 which was given 1.444 mg / day. After 30 days mice then terminated. As the following the kidneys were sliced with HE stain. The proximal convulated tubules of Wistar rat’s kidney were examined. under a microscope in five fields of view per 100 tubules. The number of degenerated tubules which each treatment was then compared with the control group and other treatments. Result: Mean renal tubular damage is the smallest in the group of P1 is 37.60 ± 20.305, whereas the average of renal tubular damage is the biggest group P3 is 71.20 ± 6.91. Kruskall-Wallis test showed significant difference (p = 0.001). Mann-Whitney test showed significant differences in the K-P1 (p = 0.009), K-P2 (p = 0.009), K-P3 (p = 0.009), P1-P3 with (p = 0.009) and P2-P3 (p = 0.009). Correlation test showed Pearson correlation value of 0.909 Conclusion: There were relationship between increasing doses of dexamethasone on the increase of the proximal renal tubule degeneration rats. Keywords: dexamethasone, 30 days, kidney histological appearance