PENGARUH KONSENTRASI dan LAMA PERENDAMAN DALAM SUPERNATAN KULTUR Bacillus sp.2 DUCC-BR-K1.3 TERHADAP PERTUMBUHAN STEK HORISONTAL BATANG JARAK PAGAR (Jatropha curcas L.)

Abstract

Jarak pagar (J. curcas L.) is a plant whose the seed can produce oil or it is known as biodiesel. J. curcas’s oil is the source of alternative oil energy that can be renewable and environmentally safe. Another ways to increase the production of J. curcas’s oil is by improving its productivity. This activity cannot be separated from the problem of supplying the seed. Stem cutting is one of the most ways to provide the seed than another way. The growth of stem cutting can be stimulated by giving ZPT that is IAA both naturally and synthetically. Bacillus sp.2 DUCC-BR-K1.3 supernatant culture that contain IAA can be one of naturally alternative hormone. The aims of this research was to study the effect of concentration and submersion period in Bacillus sp.2 DUCC-BR-K1.3 supernatant culture to the growth of J. curcas L. horizontal stem cutting. This experiment was conducted in Plant’s Biology Structure and Function Laboratory, MIPA faculty, Diponegoro University. Experimental design used in this research is Completely Random Design with Factorial pattern 5x3 by using 5 replications. The first factor was concentration, ie P0 (control), P1 (25%), P3 (50%), P3 (75%) and P4 (100%). The second factor was submerged periods, i.e T1 (1 hour), T2 (2 hours), and T3 (3 hours). The data were analyzed by using Kruskal-Wallis Test and ANOVA, if there are significantly influence, were analyzed by using Duncan multiple range test with 95% confidence intervals that is in the variable of root lenght and weight of dry root. The result of this study showed that the giving of Bacillus sp.2 DUCC-BR-K1.3 supernatant culture influence to improve the bud and root growth of J. curcas L.