UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN KAMBOJA (Plumeria alba L.)TERHADAP LARVA Artemia salina Leach DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST)

Abstract

Background: Cambodia has long been known to the Indonesian community and used as traditional medicine to treat various diseases. The usage in current amount could cause toxicity, so it is necessary to do a first screening appropriately if only the toxicity value is acute. The aims of this research is to prove the presence of toxicity potency in ethanol extract of frangipani leaf using BST methods. Method: Used 250 larvae Artemia salina Leach as test animals. As test materials are frangipani leaves ethanol extract. The observation is on the larvae that died 24 hours after administration of test materials. Based on the data, the LC 50 of ethanol extract of leaves of Plumeria alba determined by probit analysis using SPSS 16.0 for windows. Result: Mean larvae death in the concentration of 500, 200, 100, 50, and 0 µg/m consecutively were 10, 7.2, 3.6, 1.2, and 0. The result of probit analysis indicated that LC 50 value extract of frangipani leaf was 132.340 μg/ml, whereas the regression analysis results obtained from the LC 50 = 0.9388log concentration – 1.5124 Conclusion: The administering of ethanol extract of frangipani leaf, in this research, had acute toxicity potential against Artemia salina Leach larvae according to BST method. It is indicated by LC 50 value <1000 μg/ml. Key words: Plumeria alba Linn., brine shrimp lethality test, acute toxicity.