UJI TOKSISITAS AKUT EKSTRAK ETANOL BUAH ANGGUR (Vitis vinifera) TERHADAP LARVA Artemia salina Leach DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST)

Abstract

Background: Grape fruit (Vitis vinifera) is one of plants which has been known and consumed by Indonesian society. This fruit contains saponin, polifenol, flavonoid and resveratrol. The aim of this research is to know acute toxicity potential on grape fruit’s extract depend on Brine Shrimp Lethality Test (BST) method. Method: It is used 250 larvaes as test animal which is divided on 5 groups. Each group contais 10 larvaes with replication of research for 5 times. Then the extract of grape fruit got into final concentration in the media, consecutively as the group of 1,2,3,4 and 5 is 2000 µg/ml, 1000 µg/ml, 500 µg/ml, 200 µg/ml and 0 µg/ml as negative control. The result is against larvae that died 24 hours after component test was given. Through the data, LC 50 value of etanol extract of Vitis vinifera was analyzed by probit analysis using SPSS 16.0 for windows. Result: The result of probit analysis indicated that LC 50 value of fruit extract of Vitis vinifera was 648.004 μg/ml. Whereas, the result of analysis regression got equation LC50=0.239logconcentration - 0.125 Conclusion: The administering of fruit extract of Vitis vinifera, in this research, had acute toxicity potential against Artemia salina Leach larvae according to BST method. It is indicated by LC 50 value <1000 μg/ml. Key words: Vitis vinifera., brine shrimp lethality test, acute toxicity.