APPLIKASI BIOTEKNOLOGI PADA BERBAGAI JENIS SUBSTRAT YANG BERBEDA SEBAGAI UPAYA PENINGKATAN KUALITAS MEDIA KULTUR UDANG WINDU (Penaeus monodon F.)

Abstract

Amoniak yang terakumulasi di substrat merupakan masalah di budidaya udang windu (Penaeus monodon F.). Untuk mengatasi kendala tersebut, teknologi bioaugmentasi diujicobakan. Tujuan penelitian adalah untuk mengkaji pengaruh jenis substrat dan effektifitas probiotik epicin terhadap reduksi amoniak pada media pemeliharaan udang windu. Metode eksperimen laboratoris dengan Rancangan Petak Terpisah dengan dasar Rancangan Acak Kelompok dipergunakan. Perlakuan utama adalah jenis substrat (A= pasir 25% dan liat 75%; B= pasir 50% dan hat 50%; C = pasir 75% dan fiat 25%); subperlakuan adalah dosis (0 dan 1ppm) dan waktu/hari (0; 2; 4 dan 6) di sedimen serta pengulangan 3 kali. Basil penelitian menunjukkan bahwa aplikasi bioteknologi bioaugmentasi, khususnya jenis substrat tidak berpengaruh nyata terhadap reduksi amoniak media pemeliharaan udang windu. Perlakuan dosis epicin pada berbagai substrat berpengaruh sangat nyata (p<0,01) terhadap reduksi amoniak mutat hari ke 2. Dosis epicin 1,0 ppm menunjukkan kecenderungan mereduksi amoniak secara lebih optimal. Decreasing of water quality was caused by ammonia due to the ammonia is a problem in tiger shrimp (Penaus monodon F.) culture. Bioaugmentation may be can be used to solve this problem due to environmental safety. The objective of this research was to know the influence different substrat composition and effectivity of biaugmentation on ammonia reduction in culture medium of tiger shrimp. Experimental laboratoris and split plot design based on completely randomized design was applied in the research. The main treatment was the substrat substrat composition (A= sand 25% and clay 75%; B= sand 50% and clay 50%; C = sand 75% and clay 25%); as sub-treatment was epicin concentration 1{0 ppm and as a group was day (0; 2; 4; and 6) with three replication. Anova result indicated that bioaugmentation, especially substrat composition was not significantly affect on the ammonia reduction in in the culture medium of tiger shrimp. Epicin dose of 1.0 ppm and day as sub-treatment has a higly significantly affect (p<0.01) in reducing ammonia at second day and indicated the most effective to reduce ammonia in culture medium of tiger shrimp