PENGARUH PEMBERIAN SIMUNOX DOSIS BERTINGKAT TERHADAP FUNGSI FAGOSITOSIS MAKROFAG PADA MENCIT SWISS

Abstract

Background : For the preventive and curative purpose against infectious diseases and malignancies, humans need an immunostimulant to improve the quality of nonspecific or specific immune system. Simunox is one of the herbal products that may act as an immunostimulant. The study aims to investigate the effect of Simunox to the macrophage phagocytic function. Where macrophages are the main cells that play a role in non-specific defense. Methods : This was an laboratory experimental research with The Post Test-Only Control Group Design. Study population was Swiss mice that was raised in laboratories UPHP UGM Yogyakarta, sample was divided into 4 groups with simple random sampling where there are 5 mice each group. In this study, one group was given only distilled water as control and 3 treatment groups fed with various doses of Simunox 0,04 ml; 0,08 ml; 0,16 ml. Treatment was given for 7 days, on days-8 the mice were terminated and peritoneal macrophages were collected. Then, the percentage of active macrophages is calculated. Result : Descriptive analysis showed that the highest mean value was in treatment group 3. Shapiro-Wilk test, obtained the data distribution is not normal because treatment group p=0,004. Nonparametric Kruskal-Wallis test, showed significant differences between groups (p=0,003). Mann-Whitney test showed p value between two groups, the majority of results showed significant differences (p <0,05) there are K-P1, K-P2, K-P3 (p=0,009) and P2-P3 (p=0,016). Conclusion : Simunox administration was able to enhance macrophage phagocytic function compared to control group. Keywords: Simunox, macrophage phagocytic function