PENGARUH PEMBERIAN TOLAK ANGIN ANAK CAIR TERHADAP KADAR NITRIT OKSIDA (NO) PADA MENCIT SWISS

Abstract

Background : Nitric oxide is one of effectors of immune system that produced by macrophages affected by IFN-γ for intracellular destruction. Previous study proved that Tolak Angin Cair (TAC) can increase IFN-γ level. Objective : This study was aimed to analyze the effect of Tolak Angin Anak Cair (TAAC) on the NO level in swiss mice. Design and method : Experimental laboratory study with post test only control group design were performed. Forty swiss mice weighing 25-35 grams of 12 weeks old were equally divided into four groups which were : K (without any administration of TAAC), P1 (TAAC 0,125 ml), P2 (TAAC 0,25 ml), P3 (TAAC 0,5 ml). Intervention of each group were given in seven days. Then, NO level was examined using Griess reagent and read by ELISA reader. The results were analyzed using the test of normality Shapiro-Wilk continued with Kruskal-Wallis test and post hoc analysis Mann-Whitney. Result : There was a significant difference between control group and groups given TAAC (p=0,001). Significant difference occurred at the group that given TAAC 0.125 ml (p=0,019) and group given TAAC 0,25 ml (p=001) Conclusion : Tolak Angin Anak Cair can increase the NO level in swiss mice. Keywords : nitric oxide, IFN-γ, macrophages