UJI TOKSISITAS SUBKRONIS EKSTRAK VALERIAN (Valeriana officinalis) PADA TIKUS WISTAR : Studi Terhadap Gambaran Histologis Duodenum

Abstract

Background: Valerian (Valeriana officinalis) is a herbal medicine which used to insomnia therapy because it can induce sleep and one of compound is tannin. High dose of tannin cause irritation on wistar’s duodenum mucous membrane. The aim of this study was to analyze the different of duodenum histological appearance after sub chronic treatment by valerian extract between treatment groups and control group. Method: This research was an experimental study using The Post Test Only Controlled Group Design. Twenty male wistar rat were divided into 1 control group and 3 treatment groups. The treatment was given during 3 months. The control group was given aquadest. The treatment groups: P1, P2, P3 was given consecutively with 9, 18, 36 mg/rat of valerian extract. After three months, the wistar rat were terminated, duodenum were made into slides. Result: The mean score of epithelial damage in the group K=1.16; P1=1.24; P2=1.92; P3=2.20. The results of Oneway Anova test there were differences in epitelial damage score between groups (p=0,020) and the post hoc tests found significant difference between the K and P2(p=0,042), K and P3(p=0,008), and P1 and P3(p=0,013). Conclusion: Subchronic treatment of valerian extract affect duodenum histological appearance as mucous epitelial damage from desquamation, erosion, until ulceration. The calculation result of integrity score of the duodenum mucosa epithelium in threatment groups indicate more higher dosage that given, more higher epitelial damage degree. Keywords: Valerian, Valeriana officinalis, duodenum histological