EFEKTIVITAS AIR PERASAN JERUK LEMON (CITRUS LIMON BURM) 25% DIBANDINGKAN KETOKONAZOL 2% TERHADAP PERTUMBUHAN MALASSEZIA SP. PADA KETOMBE

Abstract

Background: Dandruff is a scalp disorder that caused by three interrelated factors, there are agent (Malassezia sp.), host, and environment. Lemon (Citrus limon Burm) containing d-limonene which has an antifungal. Ketoconazole is a medical therapy for people with dandruff problem that works by inhibiting ergosterol synthesis which acts to maintain the integrity of the fungal cell membrane. This study aimed to examine the effectiveness of lemon juice 25% compared with ketoconazole 2% on the growth of Malassezia sp. on dandruff. Methods: This research was done by experimental study. The samples were 30 (+) Malassezia sp. inoculated in SDA. The (+) colonies of Malassezia sp. were diluted according to the 0,5 McFarland’s standard then 0,1 cc of solution was cultivated on the SDA media supplemented with 25% lemon juice and 2% ketoconazole. After that, the media were incubated at 37C in 24-48 hours. The difference proportion of growth was analyzed by chi square test with degree of significance of p<0,05. Results: In thirty SDA media contained of 25% lemon juice, 21 were found positive growth of Malassezia sp. and 9 were found negative growth of Malassezia sp. Meanwhile in thirty media contained of 2% ketoconazole, 2 were found positive growth of Malassezia sp. and 28 were found negative growth of Malassezia sp. With chi square test, a significant difference can be seen between the effectiveness of 25% of lemon juice and 2% of ketokonazol (p=0,000). Conclusion: Twenty five percent of lemon juice was proved less effective in inhibiting the growth of Malassezia sp. invitro, compare with 2% ketoconazole. Keywords: Dandruff, Malassezia sp., 25% lemon juice (Citrus limon Burm), 2% ketoconazole.