Analisis Komunitas dan Filogeni Bakteri Bakteri Termofilik Sumber Air Panas di Wilayah Jawa Tengah

Abstract

Seven emplieons have been obtained at the first year, and need to be analyzed fit her. These PCR products were the re fragments of 16S rRNA that amplified chromosomal DNA of themmphilic bacteria from Gedongsongo, Babnniden, and Dieng hot springs. These seven amplicons have been analyzed using DOGE method. The DOGE were optimized acconimg to denaturing gradient, voltage, sad lime cleamphoresis miming The DGGE result from ELI Gedongsongo isolates revealed 12 different bands, three bands from each sample present at the same line. Seven bands have been re-PCR wad sequenced. Three bands have a phylogenetic relationship with Therms sp., and We other four bands hay,. been expected as novel bacteria. The single colony from GS (He) has a high similarity with Geordie:airs sp. according to morphological study and 168 rISNA fragment gene sequence. The Beturmden DOOR pattern showed Were are 10 different bands, Three f them have been sequenced their nucleotide and having relationship with Bacillus sp. The Mbw isolate of Baturraden single colony is Geobacillus sp There are four predominant bands in Dieng DOGE gel. Isolate AD3 from Dieng that oilmen in Sulfolobus medium showed a high similarity of their 16 rRNA gene fragment with Pseusiomonas sp. Extra cellular enzyme analysis of He, Mbw, and AD3 isolates revealed that He and Mbw have protease, amylase, and betalfidectosidase. However AD3 has any extra cellular protease. Pada ulna pertama telah diperoleh 7 amplikon UM* dianalisis rebel Imam. Produk PCR temebut bempe flagmen DNA berukuren seldlar 400 pb yang dipetoleh dad basil amplifikasi /ragmen gen 165 rRNA menggemakan templet DNA kromosom isolat bakteri temmfidik dari cumber eh pan Geelong Songo, Dieng den Baturraden. Ketujub =Mem tersebut dianalisis meaggunakan metade Denaturing Gradient Gel Electrophorus& (DGGE). DOGE dilakukan dalam berbagai kondisi =lama vatiesi gradien denaturan gel, besar tegangan den waktu yang digunalcut dalam proses elektroforcsis. Hand analisis DOGE forbad%) bagmen DNA dari selumh isolat Gedong Soup (GS) menunjuldcan adanya 12 pita, tip pita pada masingmasing isolat berada pada penal yang siMar. Toth pita DGGE dati Gedongsougo telab dianalisis man nukthotidanya, tiga pita menunjukkan kesemean culwp tiuggi &man golongan Thennus sedangkan empat pita yang lain diduga mermakan spesies ham Sam koluul nmggal dari GS (He) teridentifikan sebagai Geobacillus. Pada isolat Beturraden (BR), hoed DOGE menunjuldam adanya 10 pita. 'DP Pita yang dianalisis mem madeoddanya memmjukkan tingket kekerabatan tinggi dengan golongan Bacillus (Geobactllus), demildan pule Magma Mani maga' dati BR yaitu Mbw tames* golonganGeobacillus. Heed DOGE diktat set kawah Diem menunjukkan adanya empat pita yang cukup dominan. Seth sam kultur Dieng yang diturobthkan pada media adfolobus (AD3), daexab vanabed 9 deli gene 16S rRNA yang teramplifikesi deri DNA kromosomaya mennliki kesamaan tthggi dengan urutannukleotida can Pstudomonas sp. Hasil nji enzim eketraseluler terhadop kiiltur tunggel He Mbw, den AD3; menunjukkan bahwa He dm Mbw memiliki protease, amilase den beta¬galaktosidase. Sedengkaa AD3 limy* nodal memitiki protease ekstraseluler.