OPTIMASI PROSES EKSTRAKSI PIGMEN KAROTENOID DARI Spirulina plantesis

Abstract

Carotenoids are organic pigments that are naturally in khromoplast of plants, photosynthesis organisms such as algae (Spirulina plantesis, Dunaliella sp). It is one of the dyes used in foods and the largest group of pigments that are produced in nature. Carotenoids act as anti-oxidants in the body. Carotene is an efficient scavenger of free radicals and can significantly reduce the risk of cancer. Conducting research using the extraction method by varying the form of powder and flakes, temperature (at 30, 40, 50, 60 oC) and operating time (1, 2, 3, 4 hours). Spirulina plantesis in the extraction according to the specified variable. After that, each sample was analyzed by UV-Vis spectrophotometer SP 300. Analyzing data with ANOVA analysis and with STATISTICA 6 program. The optimum conditions obtained for the form of flake material is at a temperature of 47.1 ° C and the duration of the extraction of 3.5 hours. For the powder form of the condition of the material is at a temperature of 51.9 ° C and the duration of the extraction of 3.4 hours. Comparisons drawn between the shape of powders and flakes show that powder can produce a better yield than the form of flakes. To maintain the quality of carotenoids and prevent damage, the extraction process should be done not at high temperatures