Profil Kromatogram dan Aktivitas Antibakterial ekstrak etanol rimpang Temulawak terhadap Pertumbuhan Escherichia coli in vitro

Abstract

Background : Nowadays people tend to use natural ingredients as a form of alternative medication, one of them is Curcuma xanthorhiza Roxb, a kind of Zingiberaceae, believed as a medication for many diseases. As reported from the previous research, Curcuma xanthorhiza Roxb had an antibacterial effect. Objective : The purpose of this study was to know chromatogram profile and antibacterial activity extract etanol of Curcuma xanthorhiza Roxb rhizome for Escherichia coli growth in vitro. Method : This study were descriptive study and experimental study using the “post test only control group design”. Descriptive study, the sample was extract etanol of Curcuma xanthorhiza Roxb rhizome and the sample of experimental study was E.coli with standard strain ATCC 25922. For profile chromatogram the method which were used thin layer chromatography which the extract were put a drop on chromatograph plate then the chromatograph plate put in closed erlenmeyer with etil asetat and antibacterial activity test with dilution method,these were divided into 5 test groups and 3 control groups. The test groups treated with the different dose extract etanol of Curcuma xanthorhiza Roxb rhizome from 6,25 % v/v until 100 %. And the 3 control groups were positive control, negative control, and sample control. Result : The results of analysis using thin layer chromatography was found 5 spot. To determine MIC, after Kruskal-Wallis Test was done, the result was significant (p<0,05) and followed by Mann-Whitney Test, significant from the dose 50 % v/v. To determine MBC, after Kruskal-Wallis Test was done, the result was significant (p<0,05) and followed by Mann-Whitney Test, significant when the dose 100 %. Conclusions : Curcuma xanthorhiza have some compounds. Curcuma xanthorhiza Roxb were bacteriostatic and bactersidal for E. coli. MIC for E.coli was 50 % v/v. MBC for E. coli was 100 %. Keywords : Curcuma xanthorhiza Roxb, Escherichia coli, antibacterial activity, MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration).