PROFIL KROMATOGRAM DAN AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL RIMPANG LEMPUYANG EMPRIT( Zingiber americans Bl. ) TERHADAP BAKTERI ESCHERICHIA COLI IN VITRO

Abstract

Background : Zingiber americans is a well known advantagous herbal, containing atsiri oil that may be antibacterial. The purpose of this study was to know chromatogram profile and antibacterial activity of zingiber americans Bl for Escherichia coli in vitro. Method : This study were descriptive study and experimental study using the “post test only control group design”. In descriptive study, the sample was extract of Zingiber americans Bl and the sample of experimental study was E.coli with standard strain ATCC 25922. The method used was thin layer chromatography on which the extract were put a drop on chromatograph plate then the chromatograph plate was put in close erlemeyer with developing liquid and antibacterial activity test uses dilution method, these were divided into 5 test groups with concentrations 100% v/v, 50% v/v, 25% v/v, 12,5% v/v, 6,25% v/v and 4 control groups were positive control, negative control, sample control, and control PG5%. Result : The results of analysis using thin layer chromatography was found 6 spots. The result of antibacterial activiyty, Minimum Inhibitory Concentration (MIC) significant from the concentration 25% v/v (p=0,001) and Minimum Baktericidal Concentration (MBC) significant from the concentration 50% v/v (p=0,021). Conclusions : The results of antibacterial activity test were bacteriostatic and bacterisidal for E.coli. MIC for E.coli was 25 % v/v. MBC for E.coli was 50% v/v. Keywords : Zingiber americans Bl, Escherichia coli, antibacterial activity, MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration).