PROFIL KROMATOGRAM DAN AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL RIMPANG BENGLE (Zingiber cassumunar Roxb.) TERHADAP BAKTERI ESCHERICHIA COLI IN VITRO

Abstract

Background: Bengle (Zingiber cassumunar roxb.) was a plant which had been used by society since long time ago as a traditional herbal. It was predicted that there were anti-bacteria substances in bengle, so it might substitutes the utilization of conventional antibiotics. Objective: To find out total amount of substances in ethanol extract of bengle rhizome by its chromatogram profile and also to know its anti-bacterial to Escherichia coli. Method: This study were descriptive study and experimental study using the post test only control group design. Ethanol extract of bengle rhizome was made by maceration method with ethanol as solvent. Thin layer chromatography was used to know the numbers of compound in extract. For anti-bacteria study, Escherichia coli ATCC 25922 was used in this experiment. Each group was given extract in concentration 100%; 50%; 25%; 12,5%; 6,25%; 3,125%. The clearance of Mueller Hinton broth medias were used to determine the Minimum Inhibitory Concentration (MIC) whereas Minimum Bactericide Concentration (MBC) was determined by observing the bacteria growth on McConkey selective media. Result: There were four spots can be examined by thin layer chromatography. Activity to Escherichia coli was shown in average concentration 12,5% as minimum inhibitory concentration and average concentration 25% as minimum bactericide concentration. Conclusion: Bengle rhizome had four visible compounds. Its ethanol extract was effective for inhibiting Escherichia coli and in higher concentration, it was able to kill Escherichia coli. Keywords: ethanol extract of bengle rhizome, Escherichia.coli, anti-bacteria activity in vitro