PROFIL KROMATOGRAM DAN AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KEMUNING ( Murraya paniculata (L.) Jack.) TERHADAP BAKTERI ESCHERICHIA COLI IN VITRO

Abstract

Background : Orange Jessamine (Murraya paniculata (L.) Jack) was such traditional medicine. It had been believed to be effective for urinary tractus infection and diarrhea which almost caused by Escherichia coli. The aims of these studies were to known the chromatogram profile of Orange Jessamine (Murraya paniculata (L.) Jack) etanol extract leaf and determined both of their MIC and MIB against Escherichia coli. Method : These were such of the descriptive and experimental studies. Etanol extract prepared by maseration with etanol 40 %, then extract products were tested for their compounds using thin layer chromatography under 254 nm and 366 nm UV light. For determined antibacterial activity we used dilution method with several concentration, there were 40 %, 35 %, 30 %, 25 %, 20 %, 10 %, and 5 % continued with stricted them into Mac Conkey agar. The result data shown were analyzed with Tukey test. Result : The visible result of thin layer chromatography under UV light 254 nm there were six colour spots e.i dark blue (Rf 0,45), blue (Rf 0,58), violet (Rf 0,62), pink-violet (Rf 0,67), green (Rf 0,68), and brown (Rf 0,70). Comparisoning of their chromatography data with those reported in the literature they were found to be chemical compounds included of alcohol, high level of cethone, terpenoid, steroid, organic acid and volatile oil. Inhibitory effect had been obtained at 30 % coencentration extract, shown from the clear of sample groups with significant level ( p<0,05). Bactericidal effect was at 40 % concentration extract, shown from no growth of Escherichia coli in Mac Conkey agar with significant level ( p<0,05). Conclusion : There were six chemical compounds predictly responsible to antibacterial activity included of alcohol, high level of cethone, terpenoid, steroid, organic acid and volatile oil constituented within Orange Jessamine (Murraya paniculata (L.) Jack) etanol extract leaf. It had been also obtained as inhibitory and bactericidal agents against Escherichia coli in vitro with MIC 30 % and MBC 40 %. Keywords : Orange Jessamine (Murraya paniculata(L.)Jack), antibacterial activity, chromatogram profile.