KADAR SERUM ASPARTAT AMINOTRANSFERASE DAN ALANIN AMINOTRANSFERASE PADA TIKUS WISTAR SETELAH PEMBERIAN ASETAMINOFEN PER ORAL BERBAGAI DOSIS

Abstract

Background : Acetaminophen is an analgesic-antipyretic which has wide distribution in community. It often to be abused in over recommended therapeutic dosage. Acetaminophen is metabolized in liver. In very high dose, liver capability to inactive toxic metabolites of acetaminophen is depleted. It induced hepatocellular injury. If this condition happen, the cell’s membrane integrity would be impaired and the intracellular enzymes of the liver including Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT) would leak into the bloodstream, and as the result, the level of this enzymes might elevate higher than normal. Objective : To find out the effect of acetaminophen in various dosage to AST and ALT serum level of wistar rat. Method : The study was a true experimental study with the post test only control group design. The samples were 24 male wistar rats, with age of 4,5-6 months and weight of 200-300 gram, which were divided into 4 groups. Control group received standart diet only without acetaminophen, P1 group treated with 1200mg/kgBW of acetaminophen, P2 group treated with 2400mg/kgBW of acetaminophen, P3 group treated with 4800 mg/kgBW of acetaminophen. Retroorbitalis vein blood were taken from all groups on the 4th day after administered of acetaminophen and AST and ALT serum level were measured. Result : The result of Anova test showed that there were significant differences on AST and ALT serum level between all groups (p=0,000). Post-hoc analysis using Bonferonni test showed that K-P1 (p=0,001), K-P2 (p=0,000), K-P3 (p=0,000), P!-P2 (p=0,030), P1-P3 (p=0,000), P2-P3 (p=0,000) in AST serum and K-P1 (p=0,000), K-P2 (p=0,000), K-P3 (p=0,000), P!-P2 (p=0,000), P1-P3 (p=0,000), P2-P3 (p=0,001) in ALT serum. It showed significant differences between each groups on AST and ALT serum level (p≤0,05). Conclusion : There were significant changes of AST and ALT serum level between untreated group and treated groups. There were significant changes between groups which treated with various dosage of acetaminophen. AST and ALT serum level were elevated along with the rise of acetaminophen dosage.