Isolasi dan Karakterisasi Bakteri Karang Pendegradasi Senyawa Herbisida Glyphosate di Perairan Teluk Awur, Jepara

Setyati, Wills Ari and Subagyo, Subagyo (2003) Isolasi dan Karakterisasi Bakteri Karang Pendegradasi Senyawa Herbisida Glyphosate di Perairan Teluk Awur, Jepara. Documentation. UNIVERSITAS DIPONEGORO.

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Abstract

Coastal pollution caused by river run off of agriculture area have dangerous impact for coral reef ecosistem. The use of glyphosate¬degrading bacteria isolated from coral biofilm is alternative solution of the problem. The purposes of this research were to isolate, select and know study the kinetic of growth and substrate disposal of bacteria associated with corals which have ability to degrade glyphosate herbicide compounds at Jepara Sea. This research was conducted from August - September 2002. Sampling was carried out at Teluk Awur and Panjang island, Jepara on different coral life forms, namely massive, submassive, foliose and branching at two different stations. Isolation studies were performed at Microbiogenetics laboratory of Faculty Mathematics and Natural Sciences Diponegoro University Semarang and kinetic studies was done at Biotechnology Laboratory and Center of Phisic and Chemical Laboratory Gajah Mada University Yogyakarta. Experimental method was used in this research by location and coral life-form as source of variation. The prosedure of this research are isolation, purification, sensitivity, turbidity, degradation and kinetic tests. The result were obtained that among 233 isolates, 84 isolates (36.05%) are capable of degradating gliphosate herbicide. Kinetic studiy of growth and substrate of F.T1.27 isolat (% degradation 42.44% and sensitivity values 8 mm) showed Lineweaver - Burk linearisation of Michaelis-Menten equation Y= 0.0067 X + 4.1232 and showed that maximum growth rate (p .m maks ) was 0.2425 h saturated concentration (KO was 14.7 ppm, while the maximum effect of glyphosate concentration on the substrate removal rate (rs) was 0.0296 g/L at 50 ppm and generation time (tg) was 2.86 11-1.. Pencemaran wilayah pesisir akibat run off sungai dari daerah pertanian oleh senyawa herbisida glifosat memiliki dampak yang sangat berbahaya bagi ekosistem terumbu karang. Pemanfaatan keberadaan bakteri pendegradasi senyawa herbisida glifosat pada lapisan biofilm terumbu karang merupakan alternatif pemecahannya. Tujuan penelitian ini untuk mengisolasi, menyeleksi dan mengetahui kinetika pertumbuhan dan penggunaan senyawa glifosat bakteri yang berasosiasi dengan karang yang mampu mendegradasi senyawa herbisida glifosat di perairan Jepara. Pengambilan sampel dilakukan di Teluk Awur dan Pulau Panjang, Jepara. Sampel yang diambil terdiri atas empat jenis life-form karang pada masing - masing stasiun. Tahap isolasi dilaksanakan di Laboratorium Mikrobiogenetika MIPA, Universitas Diponegoro dan Uji kinetika dilaksanakan di Laboratorium Bioteknologi Fakultas Pertanian dan Laboratorium Kimia Fisika Pusat, Universitas Gajah Mada. Metoda penelitian yang digunakan adalah metoda eksplorasi dengan faktor lokasi dan life-form karang sebagai sumber keragaman. Prosedur penelitian meliputi isolasi, pemurnian, sensitivitas, turbiditas, degradasi dan uji kinetika. Hasil penelitian diperoleh 84 isolat atau sekitar 36,05% dari seluruh isolat (233 isolat) menunjukan kemampuan mendegradasi senyawa herbisida glifosat. Penelitian kinetika pada isolat F.T1.27 (% degradasi 42,44% dan derajat sensitivitas 8mm) menunjukan linearisasi Lineweaver - Burk tehadap persamaan Michaelis-Menten Y= 0,0067 X + 4,1232 dengan laju pertumbuhan maksimum (p maks sebesar 0,2425 jam-' dan nilai tetapan kejenuhan (Ks) sebesar 14,7 ppm. Sedangkan laju penggunaan substrat (rs) pada konsentrasi 50 ppm yaitu sebesar 0,0296 g/L glifosat dan waktu generasi sebesar (tg) 2,86 jam"'.

Item Type:Monograph (Documentation)
Subjects:S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions:Document UNDIP
ID Code:22477
Deposited By:Mr UPT Perpus 2
Deposited On:04 Oct 2010 12:04
Last Modified:04 Oct 2010 12:04

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