PERBANDINGAN EFEK ANTIBAKTERI JUS APEL (Pyrus malus) JENIS GRANNY SMITH PADA BERBAGAI KONSENTRASI TERHADAP STREPTOCOCCUS MUTANS

Abstract

Background : Streptococcus mutans(S. mutans) is the most important cariogenic bacteria. Apple contains many kind of flavonoids such as catechin, which is present in both flesh and skin. Catechin in apple might have an antibacterial effect. Objective : The aims of this study were to examine Minimum Inhibitory Concentration(MIC) and Minimum Bactericidal Concentration(MBC) of apple juice extraxted from Granny Smith varian on S. mutans in vitro and to compare the antibacterial effect in various concentration on S. mutans. Method : This study was an experimental study using the dilution method. The concentrations of the apple juice were 100%, 50%, 25%, 12,5%. MIC was determined by visual clarity of bacterial-apple juice suspension while MBC was determined by the growth of S. mutans colony on Blood agar media. Statistical analysis was done using Kruskall-Wallis Test and Mann- Whitney Test. Result : The apple juice on the concentration of 100% and 50% were significantly inhibiting bacterial growth in the bacterial-apple juice suspension. On the other hand, there was no evidence of growing S. mutans on agar plates of all tested concentrations of apple juice . Conclusions : Apple juice extracted from Granny Smith varian had a bacteriostatic effect on Streptococcus mutans and MIC on concentration of 50%. MBC could not be determined in this study. Further study is needed to know bactericidal effect of apple juice. Keywords : Streptoccus mutans, apple juice, Granny Smith, antibacterial effect.