PERBANDINGAN EFEK ANTIBAKTERIAL BERBAGAI KONSENTRASI DAUN SIRIH (Piper betle Linn) TERHADAP STREPTOCOCCUS MUTANS

Abstract

Background: Plaque is the main cause of caries, therefore the prevention of plaque forming is needed. According to some researches, catechine, one of the substances of Sirih leaves (Piper betle Linn), had the capability of inhibiting the activities of glucosiltransferase enzyme and a quality as a bactericide against Streptococcus mutans, the main cause of plaque formation. So, it decreased plaque formation on tooth surface. Objectives: The purpose of this research was to measure the antibacterial effect of Piper betle Linn infusion against Streptococcus mutans on various concentration in vitro. Method: This research was an experimental study using the dilution method. The concentration of Piper betle Linn infusion were 100%, 50%, 25% and 12,5%. Minimum Inhibitory Concentration (MIC) was determined by visual clarity of bacterial-Piper betle Linn infusion suspension. Minimum Bactericidal Concentration (MBC) was determined by the ability to prevent Streptococcus mutans from growing on Blood Agar plate. Statistical analysis was done by using Kruskal-Wallis and Mann-Whitney Test. Results: The MIC of Piper betle Linn infusion against Streptococcus mutans was 25% and the MBC of Piper betle Linn infusion against Streptococcus mutans was 100%. Conclusion: Piper betle Linn infusion was bacteriostatic at concentration 25% and bacterisid at concentration 100% for Streptococcus mutans. Keywords: Sirih leaves, Streptococcus mutans, antibacterial effect, Minimum Inhibitory Concentration, Minimum Bactericidal Concentration.