PENGARUH PEMBERIAN VAKSIN BCG TERHADAP HITUNG JUMLAH LIMFOSIT LIEN MENCIT BALB/C YANG DIBERI STRESOR RENJATAN LISTRIK DAN DIINFEKSI LISTERIA MONOCYTEGENES

Abstract

Background: Stress may cause immunity impairment especially in cellular immunity responses, while BCG vaccination may increase it. Objective: The aim of this study was emphasized on the effects of stress and combination of BCG vaccine – stress in cellular immune responses alteration through lymphocyte count in spleen. Method and subject: This study adapts laboratory experimental and Post Test Only Control Groups Design . This particular study is designed using mice as a model. The 24 female BALB/c (6 – 8 weeks old and average weight 21.48  1.70 grams). All mice then divided into three groups and received standard lab diet daily. The first group (control group / K) received no other additional treatment. While the second group (stress group / EFS) received stressed by electrical foot shock start on day 12th until day 21st and the third group (Stress + BCG group / EFS+BCG) received intra peritoneal injection of 0.1cc BCG at day 1st and 11th and stressed by electrical foot shock start on day 12th until day 21st. At day 21st, all groups were injected 0.1cc intravenously with 105 live Listeria monocytogenes (LD50 = 2x105 bacteria) and terminated at day 26th. Number of lymphocyte cel per cc and spleen weight were measured. Within group difference of data were analyzed by One Way ANOVA. Result: There are no significant differences in the lymphocyte count and spleen weight (p>0.05) among groups. The lowest number of lymphocyte count was found in the EFS group. The lowest measuring of weight spleen was found in the EFS group. Conclusion: It could be concluded that stress is immunosuppressant, while additional treatment with BCG can restore immune responses indicated by the increase of lymphocyte count in stress mice. Key words: BCG, Stress, lymphocyte count.