PENGARUH PEMBERIAN VAKSINASI BCG TERHADAP GAMBARAN HISTOPATOLOGI HEPAR MENCIT BALB/c STRES YANG TERINFEKSI Listeria Monocytogenes

Abstract

Background: Cellular immunity plays an important role in eradicating intracellular bacteria. Stress may cause cellular immunity impairment, while BCG vaccine may increase it. Objective: The aim of this study was emphasized the effects of BCG vaccine on stressed balb/c mice infected with Listeria monocytogenes in number of microabscess and hepatocyte damage. Method and subject: This study adapts laboratory experimental and Post Test Only Control Groups Design. The 24 female BALB/c divided into four groups and received standard lab diet daily. The control group received no other additional treatment. While the EFS group received stressed by electrical foot shock (EFS) start on day 12th until 21st. The BCG group receives intra peritoneal injection of 0.1cc BCG at day 1st and 11th, and the EFS+BCG group received intra peritoneal injection of 0.1cc BCG at day 1st and 11th and stressed by EFS start on day 12th until 21st. At day 21st, all groups were injected with 104 live Listeria monocytogenes intravenously and terminated at day 26th and the livers were taken to observe histopatologically. Result: There are significant difference on number of microabscess and hepatocytes damage between EFS and EFS+BCG (p<0.05). In contrast, there are no significant differences between EFS+BCG and control (p>0.05). Conclusion: It could be concluded that stress is immunosuppressant, while additional treatment with BCG vaccine can restore immune responses indicated by the lower number of microabscess and hepatocytes damage. Key words: Stress, BCG, microabscess, hepatocytes damage, L. monocytogenes.