PENGARUH PEMBERIAN JUS Persea americana Mill. TERHADAP KADAR KOLESTEROL TOTAL SERUM TIKUS JANTAN GALUR WISTAR HIPERLIPIDEMIA

Abstract

Background : Persea americana Mill., one of Indonesian herbal medicine, often used in society as traditional medicine. Persea americana Mill. contains several active regiments considered to be able to lower cholesterol levels in blood. The examination using total cholesterol, in spite of cheaper and often being done, also describes the total level of blood cholesterol. By assumption that if the level of total cholesterol is increasing from the normal limitation, so it is already in the condition of hyperlipidemic which immediately needs to be handled before it becomes severe. Thus, a study to determine the effect of stratified dose of Persea americana Mill. juice on total cholesterol serum level in hyperlipidemic rats was done. Method : An experimental study using Pre and Post Randomized Controlled Group Design was carried out to already made hyperlipidemic Wistar rats. Sample consist of 32 male Wistar rats were divided into 4 groups. They divided to one control group, which receive standard diet only, and three treatment group, which given diet containing 2 ml for treatment I, 3 ml for treatment II, and 4 ml for treatment III per day for 15 days. The analysis of total cholesterol serum level was determined using CHOD-PAP method. Data were analyzed using paired t-test and One Way Anova. The degree of significant was 5 %. Result : The administration of three dosages of Persea americana Mill. juice in the treatment groups causes the declining of total cholesterol serum level which significantly (p=0.000) to all dosages compared to control. The most effective dose was achieved in the dose of 4 ml/day because it could decrease the total cholesterol serum level which was highest than the other groups. Conclusion : The administration of 2 ml, 3 ml, and 4 ml/day of Persea amrericana Mill. for 15 days could decrease the total cholesterol serum level in hyperlipidemic rats significantly, with the most effective dose is 4 ml per day. Keyword : Hyperlipidemic, Total Cholesterol, Persea americana Mill. juice