UJI EFEKTIFITAS DAYA ANTHELMINTIK PERASAN BUAH SEGAR DAN INFUS DAUN NANAS (Ananas comosus (L.) Merr) TERHADAP Ascaridia galli SECARA INVITRO

Abstract

Background : Pineapple (Ananas comosus (L.)Merr), a kind of fruit which is often consumed by people, can be used as a traditional medicine. Pineapple fruit and leaf have many functions,such as an anthelmintic. This research was aimed to prove that fresh pineapple fruit squeeze and pineapple leaf infuse have the anthelmintic potency by comparing with NaCl 0,9 % as negatif control and piperazin citrat (as positif control), as well as comparing anthelmintic potency between pineapple fruit squeeeze and pineapple leaf infuse. Methods : This research is an experimental research with post test only controlled group design. The sample were Ascaridia galli worms, which were taken randomly. The sample were treated with pineapple fruit squeeze in concentration 10%,20%,30%,40% and Pineapple leaf infuse was in the concentration of 25%, 45 %, 65 %,85%. Positif control was piperazin citrat in the concentrationm of 0,2% and The negatif control was NaCl 0,9% Liquid. Sample from each groups was replicated three times. Data were collected through the observation of total mortality in every hour. LC100 and LT100 was calculated by probit analysis. Result : Probit analysis showed that LC100 of fresh pineapple fruit squeeze at the concentration 47,775% and LT100 of fresh pineapple fruit squeeze was 665,529 minutes. Pineapple leaf infuse had LC100 88,629 % and LT100 of pineapple leaf infuse was 1119,951 minutes. Kaplan-Meier test showed that pineapple fruit squeeze 40% was the most effectif . Pineapple fruit squeeze had effectivity less than control positif . Conclusion : Pineapple fruit squeeze and pineapple leaf infuse have the anthelmintic potency to Ascaridia galli worms in vitro. Pineapple fruit squeeze has more effective anthelmintic potency than pineapple leaf infuse. Piperazin citrat is the most effective than pineapple fruit squeeze and pineapple leaf infuse. Key Words : Pineapple fruit squeeze, pineapple leaf infuse, anthelmintic potency, Ascaridia galli.