Pengaruh Pemberian Ekstrak Biji Jintan Hitam (Nigella sativa) terhadap Jumlah Eritrosit Tikus Wistar yang Diberi Metotreksat

Abstract

Background: Methotrexate caused bone marrow depression in high doses administration, as manifested by a decrease of erythrocytes count in peripheral blood. The latest study reported that N. sativa extract could stimulate the bone marrow proliferation effectively. The objective of this study was to determined the effect of N. sativa extract on the erythrocytes count of Wistar rats administered by methotrexate. Method: This study was an experimental study with randomized pre and post test control group design. The samples were 20 male Wistar rats, randomly divided into 4 groups. Group K (received methotrexate 0.54 mg/day orally on the 16th-20th day), group P1 (received N. sativa extract 2x0.008 ml/day on the 8th-21st day and methotrexate 0.54 mg/day on the 16th-20thday), group P2 (received N. sativa extract 2x0.08 ml/day on the 8th-21st day and methotrexate 0.54 mg/day on the 16th-20thday, group P3 (received N. sativa extract 2x0.8 ml/day on the 8th-21st day and nethotrexate 0.54 mg/day on the 16th-20thday). Blood samples were taken from retroorbitae vein and the erythrocytes count were measured. Result: The Anova Test showed that there were significant differences on erythrocytes count (p<0.05). Post hoc analysis showed significant differences between K and P1 and between K and P2 (p<0.05). T-Test showed that in P1 and P2 there were significant differences of erythrocytes count between pre and post test (p<0.05). Conclusion: N. sativa extract doses 2x0.008 ml and 2x0.08 ml per day could increase the erythrocytes count in Wistar rats administered with methotrexate Keywords: Nigella sativa, methotrexate, erythrocyte