GAMBARAN HISTOPATOLOGIS DUODENUM MENCIT BALB/C PADA PEMBERIAN ARSEN TRIOKSIDA DOSIS BERTINGKAT PER ORAL

Abstract

Background: Examination on the tissue taken from an autopsy on a man exposed with arsenic through water and food showed that arsenic exist on the all of body tissue. Arsenic Trioxide may cause plasma transudation which in turn vesicle was formed under the gastrointestinal mucous. The vesicle then becomes rupture and this may cause the epithel fragment on the surface to be exfoliated. Objection: To observe the histopathological appearance of Balb/c mice’s small intestine after administered Arsenic Trioxide with stratified dosage in the oral. Method: This experimental study used Post Test Only Control Group Design, with 24 Balb/c divided into 4 groups. Group I was control (K). P1 was administered with 11 mg Arsenic Trioxide (½ lethal dosage). P2 was administered with 22 mg Arsenic Trioxide (lethal dosage). P3 was administered with 44 mg Arsenic Trioxide (2x lethal dosage). Then the small intestine were taken to be observed and made into slides with HE stain, then the mucous epithel were examined. The data were analyzed using the difference test of Kruskal-Wallis and Mann-Whitney. Result: From the slides, the epithels from group P1, P2, P3 are damaged. With Kruskall-Wallis test there are significant difference between the control group and group P1, P2, P3 with p<0,05. With Mann-Whitney test, between control and P1, control and P2, control and P3 there are significant difference. Between P1 and P3 there is also a significant difference. Conclusion: Arsenic Trioxide diet with stratified dosage in the oral had an effect on the damage of mucous epithel of Balb/c mice’s small intestine. Keywords: Arsenic Trioxide, small intestine, mucous epithel integrity