HUBUNGAN DERAJAT HISTOPATOLOGIS HEPAR MENCIT BALB/c DENGAN PEMBERIAN ARSENIK TRIOKSIDA DOSIS BERTINGKAT PERORAL

Abstract

Background : Arsenic, a heavy metal, has been used for many years in agriculture, industry, and medicine. It have been used as suicidal and homicidal poisons since the middle Ages. Acute intoxication signs include nausea, vomiting, diarrhea, which may be bloody, and abdominal pain. Arsenic Trioxide, one of hepatotoxic essence, that bio-transformed in liver and can yield the toxic metabolic product wich can damage the hepatocyte cell noted by histopathological level changes. Objective: To find the correlation of histopathological level on Balb/c mice’ liver with oral administration of graded dosage Arsenic trioxide respectivly. Method: This experimental study with post test only control group design used 24 Balb/c male mice which were divided into 4 groups. Group PI received no Arsenic Trioxide and was treated as control. Group P2 treated with 11 mg Arsenic trioxide. Group P3 treated with 22 mg Arsenic trioxide Group P3 treated with 44 mg Arsenic trioxide. Result: Outcome of Kruskal- Wallis test showed significant differences between all groups (p=0,000).Outcome of Mann Whitney test showed significant differences between alI groups. The outcome were: between P1-P2 (p=0, 014), P1-P3 (p=0,03), P1-P4 (p=0, 02), P2-P3 (p=0,014), P2-P4 (p=0,02) and P3-P4 (p=0,019). Conclusion: There were correlation of histopathological level on Balb/c mice’ liver with oral administration of Arsenic trioxide between untreated group, and Arsenic trioxide-treated groups, and between each graded dosage Arsenic trioxide-treated groups. The histopathological level changes of hepatocyte cell noted were in the form of parenchymatous degeneration, hydopic degeneration and hepatocellular necrotic. Keywords: Arsenic trioxide, liver histopathological appearance.