PENGARUH PEMBERIAN EKSTRAK Eurycoma longifolia TERHADAP DIAMETER TUBULUS SEMINIFERUS PADA MENCIT Balb/c JANTAN YANG DIBUAT STRES DENGAN STRESOR RENJATAN LISTRIK

Abstract

Background : Stress can decrease reproduction function through Hypothalamus-Pituitary-Adrenal axis. It was known that Eurycoma longifolia can increase sexual potent and spermatogenesis. The spermatogenesis process affect tubulus seminiferus diametre. Purpose : To prove that dietary Eurycoma longifolia extract can effect tubulus seminiferus diameter at male Balb/c mice with electric shock treatment. Method : This research was an laboratory experimental research with approach Paralel Group Post Test-Only Control Group Design. Samples consist of 24 male Balb/c mice, was divided into 4 groups. K was control group without treatment. P1 was group that was just given electric shock treatment. P2 was group with electric shock treatment and Eurycoma longifolia 5 mg/day. P3 was group with electric shock treatment and Eurycoma longifolia 10 mg/day. Then the testis of mice was taken to be made pathology anatomy images. The diametre of tubulus seminiferus was measured then compared each groups. Results : Most of the samples in group P4 died during research, so that were not included in analyze. Based on this research, it was found that there were no significantly the diametre of tubulus seminiferus between group K with group P1 (p=0,173), as well as between group P2 with group P3 (p=0,631). The diametre of tubulus seminiferus of group K was lower than P2 (p=0,045), as well as between group K with P3 (p=0,004).Data analyzed showed the diameter of tubulus seminiferus group P2 was higher than P1 (p=0,016) and group P3 was higher than P1 (p=0,004). Conclusion : Eurycoma longifolia extract supplementation in 5 mgs and 10 mgs dosage can increase diametre of tubulus seminiferus at male Balb/c mice stress induced with electric footshock.. Keywords : Stress, diametre of tubulus seminiferus, Eurycoma longifolia.