Perbedaan Jumlah dan Morfologi Neutrofil pada Penggunaan EDTA Konvensional dan EDTA Vacutainer

Abstract

Back Ground : The correct amount of anticoagulant influenced the laboratory examination. The correct amount on the tube of anticoagulant Conventional EDTA depends on the skill of laboratories, but anticoagulant Vacutainer EDTA has correct amount on the tube 1,5 mg/ml. Objectives : The purpose of this research was to know the difference of total and morphology neutrophils between anticoagulant Conventional EDTA and Vacutainer EDTA. Method : This study was observasional with cross sectional design. 37 sample which fulfilled the inclusives criterias : the blood uncoagulated, 3 ml blood on conventional EDTA tube, the blood flow till 3 ml on Vacutainer EDTA tube, and the blood stand for not more 2 hours. Then counts the total neutrophils by manual differential leucocyte count and the percentage of neutrophils which has been changed. Data were processed with SPSS 13.00 for Windows. The analysis was made with Paired T-Test and Wilcoxon Signed Ranks Test. Result : The mean of total neutrophils with anticoagulant Conventional EDTA was 68.68 ± 10.40, Vacutainer EDTA 66.78 ± 9.135. The mean percentage of morphology neutrophils with anticoagulant Conventional EDTA was 96.76 ± 6.233, Vacutainer EDTA was 13.14 ± 8.832. By using Paired T-Test showed that there was not significant difference for total neutrophils between anticoagulant Conventional EDTA and Vacutainer EDTA with p values 0.162. By using Wilcoxon Signed Ranks Test showed that there was significant difference morphology neutrophils between anticoagulant conventional EDTA and Vacutainer EDTA with p values 0.000. Conclucion : There was not significant difference total neutrophils between anticoagulant Conventional EDTA and Vacutainer EDTA. There was significant difference between anticoagulant Conventional EDTA and Vacutainer EDTA. Key Word : anticoagulant Conventional EDTA, anticoagulant Vacutainer EDTA, total neutrophils, morphology neutrophils.