Perbedaaan Fragilitas Eritrosit antara Subyek yang Jarang dengan yang Sering Terpapar Sinar Matahari

Abstract

Background : Ultraviolet of sun light can increase lysis of erithrocytes membrane by forming free radicals. Furthermore, this condition will give the effect of anemia. Sun light exposure occurs almost everyday especially for outdoor workers that don’t use any protector, such as sun block and umbrella. Objective : The objective of this study is to know the difference of erythrocytes fragility between subjects who were rarely and frequently exposed to sun light. Method : This research was an observasional analytic study with cross sectional approach. Speciments were taken from 60 subjects and divided into two groups. Group I were students of Medical Faculty of Diponegoro University that were rarely exposed to sun light and group II were construction workers that were frequently exposed to sun light. Three ml blood speciment from Mediana Cubiti Vein were taken from each of the subjects, then placed into tubes that filled with EDTA. The erythrocytes fragility was determined by using Sanford method of Osmotic Fragility Test (OFT) in Clinical Pathology Laboratory-Dr.kariadi Hospital Semarang. Data were analyzed using SPSS 13.0 for windows with Mann-Whitney test. Result : The mean level of erythrocytes osmotic fragility in group I was 0,341  0,039, and group II was 0,301  0,024. The result of Mann-Whitney test showed a difference between group I and group II with p values = 0,000 (p<0,05). Conclusion : There is a significant difference between subjects who were rarely and frequently exposed to sun light. Key words : Sun light, Erythrocytes fragility.