PERBEDAAN JUMLAH TROMBOSIT CARA MANUAL PADA PEMBERIAN ANTIKOAGULAN EDTA KONVENSIONAL(PIPET MIKRO) DENGAN EDTA VACUTAINER

Abstract

Background : Pasteur pipets as generally used pipets on conventional EDTA addition certainly result in significant difference of platelet count than vacutainer EDTA. This is caused by the overdose of EDTA that spuriously increases the platelet count. One of the way in reducing the possibility in making mistake with still using the conventional EDTA is the use of pipets which drop volume correctly as needed. Micropipet is a solution. Objectives : To prove that there was no difference of platelet count on conventional EDTA (micropipet) and vacutainer EDTA addition. Method : This study was an observational analytic with cross sectional approach. Thirty seven samples from both kinds of EDTA with inclusion criterias that were unstatic blood sampling, rightly 3ml blood for conventional EDTA, no partly coagulated blood, and less than 2 hours of blood storage in room temperature. Computer programme was used for processing the data. Hypothesis test was paired-samples t test. Result : The mean of platelet count on conventional EDTA (micropipet) was lower than vacutainer EDTA, that were 269594,59±29489,367/mm3 and 273918,92± 29607,036/mm3. Paired-samples t test resulted significant difference with p= 0.000. Conclusion : There was significant difference between platelet count on conventional EDTA (micropipet) and vacutainer EDTA addition. Keyword : EDTA, micropipet, vacutainer, platelet count