KERAGAMAN PROTEIN DARAH (BLOOD PROTEIN POLYMORPHISM) SAN PERANAKAN ONGOL (Bos (odious) DI JAWA TENGAH

Abstract

Genetic variation could be seen by using allele characters from certainty loci that also express as certainly gene. Phylogenetic or relationships between species could be known by measuring of genetic distance. The preliminary studies about genetic variations of Filial Ongol at Jawa Tengah at special loci have not been done yet. The aim of these studies is to know the genetic variations of Filial Ongol cattle (Hos indicus) through blood protein polymorphism using electrophoresis method. Genetic variation conducted by measuring average heterozygosity (n) and test of genetic distance between populations. Genetic relationship drawing by UPGM (unweight pair group method) cluster taxonomy method. Based on the. velocity of molecule movement we found that the Filial Ongol cattle at Jawa Tengah has a same polymorphic character, but has difference variation level. Moreover, Filial Ongol cattle from Magelang and Semarang have closeness genetic relationship. Hopefully this research can be used as biogenetic studies of Filial Ongol cattle that more useful as basic knowledge to develop indigenous breed genetic value of Indonesian cattle and also studying for relationship of genotype character and gene expression. Keragaman genetik dapat dilihat dengan menggunakan karakter Mel dad suatu focus tertentu yang merupakan ekspresi dad gen tertentu pula. Philogenetika atau perkerabatan antar spesies dapat diketahui dengan cam pengukuran jarak genetiknya (genetic distance). Studi pendahuluan tentang keragainan genetik sapi Peranakan Ongol di Jawa Tengah secara khusus pada foci terteritu belum pernah dilakukan. Penelitian ini bertujuan untuk mengetahui keragaman genetik sapi Peranakan Ongol (Sos indicus) rnelatui analisis protein darah dengan menggunakan metoda elektrophoresis. Keragaman genetik dilakulcan dengan menghitung rerata heterozigot (H) dan uji jarak genetik diantara populasi. Gambaran kluster hubungan perkerabatan dilakukan dengan UPGM (unweight pair group method) kluster taksonomi. Berdasarkan kecepatan gerakan rnolekul diperoleh hasil bahwa sapi Peranakan Ongol di Jawa Tengah merniliki karakter polimorphik yang sama narnun mempunyai tingkat keragaman yang berbeda. Lebih lanjut bahwa hubungan kedekatan perkerabatan ditunjukkan oleh populasi sapi Peranakan. Ongol dad Kabupaten Magelang dengan Kabupaten Semarang. Hasil penelitian ini diharapkan dapat menjadi suatu kajian biogenetik sapi Peranakan Ongol yang sangat berguna sebagai dasar ilmn pengetahuan untuk tujuan peningkatan mutu genetik indigenous breed sapi-sapi di Indonesia serta hubungan karakter genotype dan ekspresi gen.