UJI BANDING EFEKTIVITAS LAOS (Alpinia galanga) 2% DENGAN KETOKONAZOL 2% TERHADAP PERTUMBUHAN Malassezia furfur PADA PITIRIASIS VERSIKOLOR SECARA IN VITRO

Abstract

Background : Pityriasis versicolor (PV) is superficial cutaneous fungal infection characterized by hypopigmented or hyperpigmented macules and patches, caused by Malassezia furfur. Alpinia galanga is traditional plant usually used by common people to eliminate hypopigmented or hyperpigmented lession on skin in pityriasis versicolor. Ketokonazole is an antifungal agent, potentially in the treatment of PV. Objective : To compare the effectiveness of 2% Alpinia galanga with 2% ketokonazole in vitro to the growth of Malassezia furfur in pityriasis versicolor. Method : This study was done by laboratory experimental . As samples were 30 culture (+) of Malassezia furfur from patients of pityriasis versicolor who fulfilled clinical signs. The colonies of Malassezia furfur were diluted in sterile 0.9% NaCl to make the solution equal to 0,5 Mc Farland standard and was cultivated on the SDA media suplemented with 2% Alpinia galanga and 2% ketokonazole. Then the media were incubated at 37C for 3 days. Proportional difference was analyzed by Fisher’s exact test with p<0.05. Result : Among of 30 media of olive oil media contained with 2% Alpinia galanga, 100% were found positive growth of Malassezia furfur and 0% were found negative growth of Malassezia furfur. Meanwhile, among 30 media contained with 2% ketokonazole, 86.7% were found positive and 13.3% were negative. The result of the Fisher’s exact test is not significant (p=0,112). Conclusion : there is no significant difference between the effectiveness of 2% Alpinia galanga with 2% ketoconazole in inhibiting the growth of Malassezia furfur in pityriasis versicolor. Key words : Pityriasis versicolor, Malassezia furfur, 2% Alpinia galanga, 2% ketokonazole