UJI BANDING EFEKTIFITAS PERASAN BUAH BELIMBING WULUH (Averrhoa bilimbi) 6% DENGAN KETOKONAZOL 2% SECARA INVITRO TERHADAP PERTUMBUHAN Candida albicans PADA KANDIDIASIS VAGINALIS

Abstract

Background: Vaginal candidiasis is fungal infection usually caused by Candida albicans. Ketoconazole is an effective antifungal agent in the treatment of candidiasis vaginalis by blocking ergosterol synthesis as an important component for membrane integrity of fungal cell.Averrhoa bilimbi is a traditional drug which contain atsiri oil that has antimicrobial activity to Candida albicans. Objective: To compare the effectiveness of squeeze of 6% Averrhoa bilimbi versus 2% ketoconazole in vitro to the growth of Candida albicans in vaginal candidiasis. Method: This study used an experimental design. Samples were positif Candida albicans inoculation in Sabouraud Dextrose Agar (SDA) from patients of vaginal candidiasis with clinical founding and gram staining. Colonies of Candida albicans were diluted in 0,9% NaCl to make the solution equal to 0,5 Mc Farland. Then 0,1 cc of it was taken and cultivated into SDA medium supplemented with squeeze of 25% garlic and supplemented with 2% ketoconazole. Then the medias were incubated at 370C for 1-2 days, and the growth was observed on the second day. The difference proportion of growth was analyzed by fisher’s exact test with degree of signifance ( p) < 0,05. Result: 30 media of SDA which contained squeeze of 6% Averrhoa bilimbi ,30(50%) were found (+)/positive for Candida albicans growth .Meanwhile,30 media which contained 2% ketoconazole,24(40%) were found positive and 6(10%) were found negative.The result of the chi-square test is significant p=0,000. Conclusion: Squeeze of 6% Averrhoa bilimbi is not effective as 2% ketoconazole in inhibiting the growth of Candida albicans in vaginal candidiasis . Key words : Vaginal candidiasis, Candida albicans, Averrhoa bilimbi, Ketoconazole.