UJI BANDING EFEKTIVITAS EKSTRAK RIMPANG LENGKUAS (Alpinia galanga) 10% DENGAN KETOKONAZOL 2% SECARA IN VITRO TERHADAP PERTUMBUHAN Candida albicans PADA KANDIDIASIS VAGINALIS

Abstract

Background: Vaginal candidiasis is an infection of the vagina caused by Candida albicans. Ketokonazol is an antifungal agent that used in the treatment of candidiasis by inhibiting the cytochrome P-450 14α-demethylase which function in the synthesis of ergosterol, an important compound of fungal cell membrane synthesis. Lengkuas (Alpinia galanga) contains antimicobial diterpene and eugenol which have the antifungal activity against Candida albicans. This study compared the effectiveness of 10% Alpinia galanga’s rhizome extract versus 2% ketoconazole in vitro to the growth of Candida albicans in vaginal candidiasis. Method: This study was done by a laboratory experimental design. Samples were positif Candida albicans innoculation in Sabouraud Dextrose Agar (SDA) from patient of vaginal candidiasis with clinical founding and gram staining. The colonies of Candida albicans were diluted in steril 0,9% NaCl to make the solution equal to 0,5 Mc Farland. As many as 0,1 cc of solution was cultivated on the SDA supplemented with 10% Alpinia galanga’s rhizome extract and 2% ketoconazole, then the media were incubated at 370 C for 2 days. The difference proportion of growth was analyzed by Fisher’s exact test with p<0,05. Result: Thirty (100%) media of SDA which contained 10% Alpinia galanga’s rhizome extract were found negative Candida albicans growth. Thirty media which contained 2% ketoconazole, 6(20%) were found positive and 24(80%) were found negative. The result of the Fisher’s exact test is significant (p<0,05). Conclusion: Ten percent Alpinia galanga’s rhizome extract was more effective than two percent ketoconazole in vitro to the growth of Candida albicans in vaginal candidiasis. Key words : vaginal candidiasis, Candida albicans, Alpinia galanga, Ketoconazole.